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KMID : 1007320080140030227
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Journal of the Korean Society of Menopause
2008 Volume.14 No. 3 p.227 ~ p.237
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The Relationship between Tumor Necrosis Factor (TNF)-TNF Receptor (TNFR) Gene Polymorphisms and the Production of TNF and Soluble TNFR (sTNFR) by Whole Blood Cells in Korean Postmenopausal Women
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Chun Sung-Wook
Kim Hoon
Kim Yong-Jin
Jee Byung-Chul
Ku Seung-Yup
Suh Chang-Suk
Kim Seok-Hyun
Choi Young-Min
Moon Shin-Yong
Kim Jung-Gu
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Abstract
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Objectives: To investigate the association between tumor necrosis factor (TNF)-TNF receptor (TNFR) gene polymorphisms and the production of TNF and soluble TNFR (sTNFR) by whole blood cells in Korean postmenopausal women.
Methods: The TNF-? G(?308)A, C(?857)T, C(?863)A, T(?1031)C, TNF-? A252G, TNFR1 A36G, and TNFR2 T676G, A1663G and C1690T polymorphisms were analyzed by polymerase chain reaction (PCR)- restriction enzyme fragment length polymorphism (RFLP) analysis, Taqman assay and direct DNA sequencing in 67 Korean postmenopausal women. The production of TNF-?, TNF-?, sTNFR1, and sTNFR2 by lipopolysaccharide (LPS)-stimulated whole blood cells were also measured.
Results: The production of TNF-? and TNF-? by whole blood cells were not statistically different according to the TNF-? G(?308)A, C(?857)T, C(?863)A, T(?1031)C and TNF-? A252G polymorphisms, and haplotype genotypes of these polymorphisms. There were no significant differences in the production of sTNFR1 and sTNFR2 by whole blood cells in relation to the TNFR1 A36G, and TNFR2 T676G, A1663G and C1690T polymorphisms, and haplotype genotypes of these polymorphisms.
Conclusion: The TNF and TNFR gene polymorphisms do not affect the production of TNF-?, TNF-?, sTNFR1, and sTNFR2 by LPS-stimulated whole blood cells.
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KEYWORD
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TNF, sTNFR, Gene polymorphisms, Whole blood cells
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